IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like peptides and HAE (HAESA)-like receptors regulate corolla abscission in Nicotiana benthamiana flowers

Abstract Background Abscission is an active, organized, and highly coordinated cell separation process enabling the detachment of aerial organs through modification cell-to-cell adhesion breakdown walls at specific sites on plant body known as abscission zones. In Arabidopsis thaliana , floral cauline leaves regulated by interaction hormonal peptide INFLORESCENCE DEFICIENT IN ABSCISSION (IDA), a pair redundant receptor-like protein kinases, HAESA (HAE) HAESA-LIKE2 (HSL2), SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) co-receptors. However, functionality this signaling module has not yet been demonstrated in other species. Results The expression NbenIDA1 homeologs receptor NbenHAE.1 was supressed base corolla tube inoculation two virus-induced gene silencing (VIGS) constructs Nicotiana benthamiana . These suppression events arrested but did produce any obvious effect growth. VIGS plants retained higher number corollas attached to flowers than control plants, observation related greater breakstrength. arrest associated with preservation parenchyma tissue that, contrast, virtually collapsed normal corollas. viral vector construct that increased NbenIDA1A negatively affected growth inoculated accelerating timing both senescence abscission. heterologous ectopic overexpression citrus CitIDA3 AtIDA N. alter standard phenotype suggesting proteolytic processing machinery unable yield active peptides. Conclusion Here, we demonstrate encoding small peptides IDA -like family cellular awhere adventitious AZ should be formed and, therefore, flowers. Altogether, our results provide first evidence supporting notion IDA-HAE/HSL2 conserved angiosperms.